Adaptability - Project Description





ADAPTABILITY Project Description
Workpackage 1 Workpackage 1.1 Workpackage 1.2 Workpackage 1.3 Workpackage 1.4 Workpackage 1.5 Workpackage 2 Workpackage 2.1 Workpackage 2.2 Workpackage 2.3 Workpackage 3 Workpackage 3.1 Workpackage 3.2 Workpackage 4 Workpackage 4.1	Workpackage 2.1. Investigations on the basis of alloenzyme markers. Analyses of seed samples (megagametophyte/embryo)
	Start date: 1^st month	Partners responsible: UNI GOE
	Completion date: 27^th month	Other partners involved

	Objectives
	To test the hypothesis that the very rapid adaptation to the climatic conditions during sexual reproduction is based on genetic selection processes occuring before or after the fertilisation, the genotype structure of female gametophytes and embryos will be surveyed to compare these genetic structures between seed progenies from identical crosses in different climatic environments

	Description on the work
	In the first phase of the study the genetic structure of seeds from controlled crosses performed by P3 in different climatic environments will be analysed. By comparing the genotype of female gametophytes and embryos it is possible to identify the genotype of the respective pollen. The genotypic structure will be determined by means of numerous isozyme gene markers. Putative selection processes in these ontogenetic stages may refer directly or indirectly (via genomic association) to these gene markers, but it is intended to choose also those isozymes which are involved in frost and/or drought resistance mechanisms. Based on genotype comparisons between seed progenies from the same parents but produced in different environments, an assessment of actual selection in the early stages of gametophyte or embryo development will be made. This part of the work shall be supplemented by the investigations with molecular markers performed by participants P2 and P5

	Deliverables
	Isoenzymes Database 0-28 Manuscript for publication

	Milestones
	1° year: Comparison of genetic structures from progenies available in the Beginning of the project 3° year: Comparison of genetic structures from progenies available after 18 month Joint publication combining data from the other working groups.

© 2001 ISOGEN