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ADAPTABILITY Project Description | |||
Workpackage 1 Workpackage 1.1 Workpackage 1.2 Workpackage 1.3 Workpackage 1.4 Workpackage 1.5 Workpackage 2 Workpackage 2.1 Workpackage 2.2 Workpackage 2.3 Workpackage 3 Workpackage 3.1 Workpackage 3.2 Workpackage 4 Workpackage 4.1 |
Workpackage
3.1. Environmental effects on methylation of DNA and translation of phytochrome genes |
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Start date: 1st month | Partners responsible: NISK | ||
Completion date: 36th month | Other partners involved: TUM | ||
Objectives | |||
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Description on the work | |||
For objective 1, DNA is extracted from seedlings and small spruce plants with clear differences in adaptation to cold and light treatment. Since it is well known that environmental factors affects the methylation of DNA the extracted DNA will be digested with methylation sensitive and insensitive restriction enzyme isoschizomers, amplified with a PCR technique described for this type of analyses, and analysed using agarose gel electrophoresis. Several previous results suggest that phytochrome may be a key factor in the adaptation process. Two different cDNA's encoding phytochromes has been isolated from Norway spruce. These will be used for objective 2, to follow the expression of the corresponding genes during bud set in RNA hybridisation experiments. The group has experience to isolate high quality RNA from different tissues of this recalcitrant plant and has also equipment to quantify the signals. To achieve objective 3 the sequence of the two phytochrome cDNAs will be utilised to design a peptide to be used to generate specific antibodies. These antibodies is then used in immunolocalization experiments to follow the localisation pattern and pattern of accumulation during the development of vegetative buds | |||
Deliverables | |||
Two papers in international scientific journals with referees will be published from this part of the project. One will deal with the DNA methylation and the other will deal with the effects on transcription and translation of the two phytochrome genes; 0-36 | |||
Milestones | |||
1° year:
2° year:
3° year:
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© 2001 ISOGEN |
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