ADAPTABILITY Project Description
Workpackage 1 Workpackage 1.1 Workpackage 1.2 Workpackage 1.3 Workpackage 1.4 Workpackage 1.5 Workpackage 2 Workpackage 2.1 Workpackage 2.2 Workpackage 2.3 Workpackage 3 Workpackage 3.1 Workpackage 3.2 Workpackage 4 Workpackage 4.1	Workpackage 2.3. Investigations on the basis of EST-markers
	Start date: 1st month		Partners responsible: TUM
	Completion date: 36th month		Other partners involved
	Objectives
	The influence of different environmental signals (photoperiod, temperature, and drought) on the sexual reproduction of Norway spruce will be investigated in full-sib families at the stage of viable seeds as well as corresponding field test populations at the stage of seedlings. These full-sib families which were produced from the same set of parents in different crossing environments will be characterized by means of stress related EST markers in order to study the existence of genetic selction and “after effects”
	Description on the work
	Based on the different crosses (1989,1993, and 1998; delivered from partner P3), a genetic characterization of both the outdoor and indoor progenies will be performed. For this purpose, DNA will be extracted from embryo seed samples. Genotyping will be done using an expanding set of newly-developed EST markers. Such codominat EST markers (7 were designed by P2 up to date) resulted from our ongoing large-scale sequencing project using an elicitor-induced cDNA library from Norway spruce. A pilot population study previously indicated that our new EST markers are subject of genetic selection for example in response to long-term heavy metal pollution (Rieger et al., 1999). Various genetic parameters will be calculated on the basis of allele frequencies (see topic “Deliverables”). Data will be compared between both the indoor and outdoor embryo progeny subset of each full-sib family. Using the EST markers above mentioned, genetic inventories will be furthermore performed in field populations which are derived from the indoor and outdoor progenies and grew up under different environmental conditions
	Deliverables
	Compilation of multilocus-genotypes, investigation of the data by means of standard population genetic tools, i.e. genetic variation within and among families, adaptive capacity and adaptive potential Study of reference structures, characterization of genetic loads and test for selection of specific alleles/genotypes under the environmental conditions used Publication of results in a reviewed journal Analyses on methylation of mitotic cycline and some other genes as and publication of results in a reviewed journal in WP 3.1.
	Milestones
	1st year: genetic inventory of the full-sib families at the stage of viable seeds 2nd year: genetic inventory of the full-sib families at the stage of field populations; pilot study on the mehylation of coding genes, dot-blot-hybridisation of 140 cDNA clones using plant RNA isolated from different stress treatments, starting with RNA fingerprints from different stressed tisues 3rd year: finishing genetic inventories in seed and seedling samples; continuation of methylation assays and RNA fingerprints. Cooperation in the validation and exploitation of results
© 2001 ISOGEN