ADAPTABILITY Project Description
Workpackage 1 Workpackage 1.1 Workpackage 1.2 Workpackage 1.3 Workpackage 1.4 Workpackage 1.5 Workpackage 2 Workpackage 2.1 Workpackage 2.2 Workpackage 2.3 Workpackage 3 Workpackage 3.1 Workpackage 3.2 Workpackage 4 Workpackage 4.1	Workpackage 1.1. Crossing experiments with the same genotypes under different environments (climates) in a phytotron
	Start date: Month 1		Partners responsible: NISK
			Other partners involved
	Objectives
	The objective will be to obtain seeds from identical crosses made under long days and high temperatures, long days and low temperatures, short days and high temperatures, and short days and low temperatures. Seedlings from these seeds will be tested to separate the impacts of photoperiod and temperature given during sexual reproduction of the mother clones on the progeny performance in adaptive traits
	Description on the work
	Grafted clones will be induced to flower in June 2000, by means of heat and gibberellin treatments in a greenhouse at Biri nursery. The grafts to be used as mothers will be transported from the nursery to the phytotron at the University of Oslo. Two grafts from each mother clone will be grown inside each of 8 phytotron rooms. The experimental unit, which will be replicated twice, will be four phytotron rooms, each room with a unique combination of photoperiod and temperature. Thus, the entire experiment comprises 8 phytotron rooms, 4 clones, and 16 grafts per mother clones (four grafts per mother per crossing treatment). Both temperature levels and photoperiod levels will be dynamically varied over this period in order to mimic what happens under natural conditions along latitudinal and altitudinal gradients. Four non-related clones with abundant female flowers (mothers) will be crossed with four non-related fathers in the phytotron (June 2001). The pollen to be used will be extracted, cleaned and dried at Biri nursery, and then used in independent pair-crosses, pollinating the mother grafts in the phytotron rooms. Thus, the outcome will be seeds from four non-related full-sib families, crossed under four different contrasting combinations of temperature and photoperiod. Ripe cones will be collected on the grafts, most likely after at least five months in the phytotron rooms. The seeds will be extracted and cleaned
	Deliverables
	Seeds for progeny tests in a phytotron (1.2), and for alloenzyme and DNA marker characterisation will be delivered by November 2001; 0-18
	Milestones
© 2001 ISOGEN