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This partner will provide seeds
and plant material from full-sib families of Norway spruce produced after
controlled pollination and seed production in different environments to the
other
![]() The first year we will do controlled pollination on Norway spruce grafts which are kept in eight phytotron rooms until the seeds are developed. Temperature and day length will be regulated in these rooms so that two rooms will have one of four climatic treatments, combining high and low temperatures and short and long days. Altogether five female and five male parents will be involved in these crosses. We will later test the seedlings from the seed lots produced from these full-sib families in artificial freezing tests. Seeds will also be distributed to the other project participants for isozyme and molecular genetic analyses.
In artificial freezing tests performed with
two-year-old seedlings from families and provenances ten years ago we selected
seedlings that survived temperatures of -6°C, -8°C and -12°C.
These seedlings were planted in a field test in 1993 together with seedlings
that had not been exposed to the low temperatures. In this experiment
measurements will be made of bud phenology, freezing tests of twigs will
be performed both in the spring and in the autumn and
A field trial was recently established with seedlings from seeds collected in spruce stands in Norway planted with provenances from Germany, Austria and Poland, and with seedlings from direct import of the same provenances. We will make measurements of the annual growth rhythm of this plant material to verify the early observations from freezing tests that a seemingly fast adaptation has taken place after one generation in the northern environment.
In a three-step approach we will test if
methylation of DNA is a possible molecular mechanism explaining the environmental
preconditioning phenomenon. The first step includes the use of northern and
immuno-blot analyses of phytochromes O and P. Seedlings of one full-sib family
will be studied under active growth and during growth cessation. If these
experiments reveal different
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